Near-field effects on coherent anti-Stokes Raman scattering microscopy imaging
نویسندگان
چکیده
منابع مشابه
Polarization coherent anti-Stokes Raman scattering microscopy.
We report polarization coherent anti-Stokes Raman scattering (P-CARS) microscopy that allows vibrational imaging with high sensitivity and spectral selectivity. The nonresonant background signals from both Raman scatterers and the solvent are efficiently suppressed in P-CARS microscopy. We demonstrate P-CARS imaging of unstained cells based on the contrast of the protein amide I band.
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Sir Chandrasekhara Venkata Raman was justifiably excited when he noticed that when a monochromatic light beam is passed through a simple transparent liquid, different colors can be detected in the scattered light. Raman called this frequency-shifted scattered light ‘a new type of secondary radiation’, because he realized that the phenomenon he discovered was caused by a molecular property diffe...
متن کاملRefractive effects in Coherent Anti-Stokes Raman Scattering (CARS) Microscopy
Coherent anti-Stokes Raman scattering (CARS) microscopy with high sensitivity, and high three dimensional resolution, has been developed for vibrational imaging of chemical species [1]. Due to the coherent nature of the CARS emission, it has been reported that the Epi-CARS (E-CARS) and Forward-CARS (F-CARS) signals detection depends on the size and shape of the sample [2,3]. In this paper, we i...
متن کاملRefractive effects in coherent anti-Stokes Raman scattering microscopy.
Coherent anti-Stokes Raman scattering (CARS) microscopy with high sensitivity and high three- dimensional resolution has been developed for the vibrational imaging of chemical species. Due to the coherent nature of the CARS emission, it has been reported that the detection of epi-CARS and forward-CARS (F-CARS) signals depends on the size and shape of the sample. We investigate theoretically and...
متن کاملNumerical study of effects of light polarization, scatterer sizes and orientations on near-field coherent anti-Stokes Raman scattering microscopy.
We employ the finite-difference time-domain (FDTD) technique as a numerical approach to studying the effects of polarization, scatterers' sizes and orientations on near-field coherent anti-Stokes Raman scattering (CARS) microscopy imaging. The results show that to acquire better image contrast and larger near-field CARS signals, the scatterers with diameters of less than three-eighths of the pu...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2007
ISSN: 1094-4087
DOI: 10.1364/oe.15.004118